FAM168A Knockout cell line(T98G)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

FAM168A Knockout cell line(T98G) Order

Catalog#

YKO-HJ028

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FAM168A Knockout cell line (T98G)

Morphology

Fibroblast，adherent

Passage ratio

1:2-1:4

Species

Human

Gene Name

FAM168A

Gene ID

23201

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate 100 mM Solution

Target region

Knockout region: Exon 4 (based on transcript FAM168A-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:T98G			STR Info (Cell bank) Cell Line:T98G [T98-G]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	12		10	12
D13S317	13	13		13	13
D7S820	9	10		9	10
D16S539	13	13		13	13
VWA	17	20		17	20
TH01	7	9.3		7	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	10	12		10	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FAM168A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the T98G cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FAM168A gene knockout FAM168A Knockout cell line (T98G) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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KO Cell Lines

FAM168A Knockout cell line (Hela)

Organism: Human

Gene Name: FAM168A

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inquiry More details
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Organism: Human

Gene Name: FAM168A

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inquiry More details
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FAM168A Knockout cell line(T98G)

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FAM168A Knockout cell line(T98G) Order

Detailed Product Information

Instruction

Related Popular Products

Wild-type Cell Lines

Human Glioblastoma Cell Line(T98G) More details Order

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit More details Order

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium More details Order

KO Cell Lines

FAM168A Knockout cell line (Hela) inquiry More details

FAM168A Knockout cell line (HCT 116) inquiry More details

FAM168A Knockout cell line (HEK293) inquiry More details

Related Popular Products

Wild-type Cell Lines

Human Glioblastoma Cell Line(T98G)

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

KO Cell Lines

FAM168A Knockout cell line (Hela)

FAM168A Knockout cell line (HCT 116)

FAM168A Knockout cell line (HEK293)

Gene Editing Services

EZ-editor™ Products

Contact us✖

Human Glioblastoma Cell Line(T98G)
More details Order

High-performance Mouse Genotyping Kit
More details Order

EZ-cryo™ Cell Freezing Medium
More details Order

FAM168A Knockout cell line (Hela)
inquiry More details

FAM168A Knockout cell line (HCT 116)
inquiry More details

FAM168A Knockout cell line (HEK293)
inquiry More details